A. Fatty Acyls

1. Fatty Acids

Fatty acids are analyzed by GC-MS and fully quantitated by comparison with authentic standards. Currently, the fatty panel consists of 33 fatty acids, from C12 to C26 including all saturated fatty acids from 12 to 26 carbons, mono- and poly-unsaturated fatty acids and their positional isomers.

a) Free (non-esterified) Fatty Acid Panel

b) Total (sum total of esterified and non-esterified) Fatty Acid Panel

2. Eicosanoids

Eicosanoids and N-acylethanolamines are analyzed by UPLC-MS/MS and are fully quantitated by comparison with authentic standards.

a) Comprehensive Eicosanoid Panel

This is a global eicosanoid profile consisting of over 150 eicosanoids.

            b) 10-Eicosanoids Panel

This is a pre-defined set of 10 eicosanoids (contact us for details of available sets).

c) N-acylethanolamines

B. Glycerophospholipids

    For glycerophospholipids, we offer several approaches depending on the depth of analysis that is required.

1. Phospholipid Class Panel

The class panel consists of a quantitative phospholipid class scan that includes phosphatidylserine,        phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidic acid, phosphatidylglycerol and all lyso derivatives of these. We can quantify all of these classes or limit to only the ones that you select.

2. Comprehensive Phospholipid Panel

The comprehensive panel analyzes all individual isobaric species contained within each class. For routine analysis we provide normalized semi-quantitative data on all isobaric species. The semi-quantitative results allow for direct comparison between samples or treatments. On special request, we can also quantitate the isobaric species based on existing standards.

The highest level of analysis employs sophisticated acquisition protocols based on MS/MS/MS processes that will provide the exact fatty acid composition of each isobaric species. This protocol is the most elaborate and will be performed on special request.

C. Glycerolipid Panel

The glycerolipid panel provides data on all individual triacylglyceride and diacylglyceride molecular species that differ in their fatty acid composition. Due to the limited availability of quantitative standards, we recommend glycerolipids profiling scans at the semi-quantitative level, which allows direct comparison between samples or treatments. On special request, we can also quantitate the isobaric species based on existing standards. 

D. Sphingolipids

The sphingomyelin panel and ceramide panel profile panel provides data on all individual molecular species. We recommend these profiling scans at the semi-quantitative level, which allows direct comparison between samples or treatments. The sphingoid bases consist of sphingosine, sphinganine and sphingosine-1-phosphate. These measurements are performed fully quantitatively.

1. Sphingomyelin Profile Panel

2. Ceramide Profile Panel

3. Sphingoid bases

E. Cholesterol Ester Panel

For the analysis of cholesterol esters, we use a MS protocol that yields profiling data on all individual cholesterol ester molecular species based on the associated fatty acids.  We recommend cholesterol ester profiling scans at the semi-quantitative level, which allows direct comparison between samples or treatments.  On special request, we can also quantitate the molecular species based on existing standards.  

F. Sterols

Our basic sterol panel consists of 14 sterol and oxysterol metabolites; however, our method is easily adaptable to include additional metabolites of interest. Sterol and oxysterol profiling is performed fully quantitatively. 

1. Free (non-esterified) Sterol Panel

2. Total (sum total of esterified and non-esterified) Sterol Panel

G. Consultation

Dr. Oswald Quehenberger is available to provide additional consultation for projects, including more detailed lipid metabolism information, sample preparation instructions, experimental design and setup, and data interpretation. Consultation is billed at an hourly rate.