Important COVID-19 Update:

Responding to the outbreak of COVID-19, we are working to do our part to ensure the safety of our community. We are complying with all federal, state and local public health directives, including working remotely to the extent possible to minimize on-site presence. As a result, our work capacity is temporarily reduced and prioritized to maintain essential and critical research. For specific questions, please contact Milda Simonaitis at msimonaitis@health.ucsd.edu.

Services

The services we offer are outlined below. Included in the analysis are all sample extraction procedures, data acquisition and data processing. For every analytical service performed, a report will be generated and sent to the client. Approximate costs are available on our pricing page. For a detailed cost assessment, please complete a quote request.

We provide broad lipidomics analyses of all the major mammalian lipid categories including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids and sterol lipids. We perform fully quantitative or semi-quantitative analyses using gas chromatography-mass spectrometry (GC-MS) or liquid chromatography (LC) -MS/MS platforms. Our analytical approach preserves the lipid structure, identifies the lipid backbone and yields information on the fatty acid composition of complex lipids.

Analytical Services Offered

Fatty Acyls

1. Fatty Acids

Fatty acids are analyzed by GC-MS and fully quantitated by comparison with authentic standards. Currently, the fatty panel consists of 33 fatty acids, ranging in chain length from 12 to 26 carbons, and includes all saturated fatty acids, mono- and poly-unsaturated fatty acids and their positional isomers

a) Free (non-esterified) Fatty Acid Panel

b) Total (sum total of esterified and non-esterified) Fatty Acid Panel


2. Eicosanoids

Eicosanoids and N-acylethanolamines are analyzed by UPLC-MS/MS and are fully quantitated by comparison with authentic standards. The Comphrehensive Eicosanoid Panel is a global eicosanoid profile, presently consisting of over 150 eicosanoids. The 10-Eicosanoid Panel is a client-defined set of 10 eicosanoids (contact us for a list of eicosanoids to choose from).

a) Comprehensive Eicosanoid Panel

b) 10-Eicosanoid Panel

c) N-acylethanolamines

Glycerophospholipids

For glycerophospholipids, we offer several approaches depending on the depth of analysis that is required.

1. Phospholipid Class Panel

The class panel consists of a quantitative phospholipid class scan that includes phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidic acid, phosphatidylglycerol and all lyso derivatives of these. We can quantify all of these classes or limit to only the ones that you select.

2. Comprehensive Phospholipid Panel

The comprehensive panel analyzes all individual species contained within each class and employs sophisticated acquisition protocols that will provide the exact fatty acid composition of each lipid molecular species. For routine analysis, we provide relative abundances that are measured as semi-quantitative data and are normalized to internal standards. The semi-quantitative results allow for direct comparison between samples or treatments. On special request, we can also quantitate the isobaric species based on existing standards.

Glycerolipid Panel

1. Comprehensive Glycerolipid Panel

The glycerolipid panel provides data on all individual triacylglyceride and diacylglyceride molecular species that differ in their fatty acid composition. Due to the limited availability of quantitative standards, we recommend glycerolipids profiling scans at the semi-quantitative level, which allows direct comparison between samples or treatments. On special request, we can also quantitate all molecular species based on existing standards.

Sphingolipids

1. Sphingomyelin Profile Panel

2. Ceramide Profile Panel

3. Comprehensive Sphingolipid Panel

4. Sphingoid bases

The individual sphingomyelin and ceramide panels provide data on all individual molecular species. We recommend these profiling scans at the semi-quantitative level, which allows direct comparison between samples or treatments. The Comprehensive Sphingolipid Panel consists of both the Sphingomyelin Profile Panel and the Ceramide Profile Panel. The sphingoid bases include sphingosine, sphinganine, sphingosine-1-phosphate and sphinganine-1-phosphate. These measurements are performed fully quantitatively.

Cholesterol Esters

1. Cholesterol Ester Panel

For the analysis of cholesterol esters, we use a MS protocol that yields profiling data on all individual cholesterol ester molecular species based on the associated fatty acids. We recommend cholesterol ester profiling scans at the semi-quantitative level, which allows direct comparison between samples or treatments. On special request, we can also quantitate the molecular species based on existing standards.

Sterols/Oxysterols

1. Free (non-esterified) Sterol Panel

2. Total (sum total of esterified and non-esterified) Sterol Panel

Our basic sterol panel presently consists of over 20 sterol and oxysterol metabolites; however, our method is easily adaptable to include additional metabolites of interest. Sterol and oxysterol profiling is performed fully quantitatively.

Complex Lipid Panel

1. Complex Lipid Panel

Our Complex Lipid Panel includes the Comprehensive Phospholipid Panel, the Comprehensive Glycerolipid Panel, the Comprehensive Sphingolipid Panel, and the Cholesterol Ester Panel.

Sample Preparation

1. Extraction

2. Homogenization (of solid tissue)

3. Protein Analysis

Consultation

Dr. Oswald Quehenberger is available to provide additional consultation for projects, including more detailed lipid metabolism information, sample preparation instructions, experimental design and setup, and data interpretation. Consultation is billed at an hourly rate.

Major Equipment

SCIEX 6500 triple quadrupole linear ion trap mass spectrometer (6500QTRAP) with Waters Acquity Ultra-High Performance Liquid Chromatography (UPLC) system

SCIEX 4000 triple quadrupole linear ion trap mass spectrometer (4000QTRAP) with Shimazu High Performance Liquid Chromatography (HPLC) system

ThermoFisher Q Exactive mass spectrometer with Vanquish UHPLC system

Agilent 5975 gas chromatograph with Mass Selective Detector (GC/MSD)

Experimental Design

For consultation on experimental design and minimum sample requirements, please contact our director, Dr. Quehenberger, BEFORE SENDING SAMPLES.

Below are some suggested study design guidelines (not mandatory).